We are honoured to have professor Stephen Bustin (University of London, UK), professor Leendert Looijenga (Rotterdam, the Netherlands)
and dr Filip Pattyn (Ghent, Belgium) as invited speakers. We believe the presence of these eminent speakers and authorities in the field
will stimulate real-time PCR users to attend and actively participate to this unique one day symposium. Stephen Bustin will give an eloquent
talk on quality assessment of real-time PCR experiments, Leendert Looijenga will introduce us in the fascinating world of non-coding RNAs,
and Filip Pattyn will present a high-throughput primer design pipeline.
We sincerely hope you will enjoy this program, and are looking forward to meet you in Ghent!
| 9:00 - 9:30 |
Registration and coffee |
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Opening presentation |
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Jo Vandesompele, Center for Medical Genetics Ghent |
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Session 1: Optimization & Validation |
| 9:30 - 10:00 |
Essential precepts for unambigous qPCR data reporting |
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Stephen Bustin, Barts and the London School of Medicine and Dentistry, London, UK (invited speaker) |
| 10:00 - 10:20 |
Requirements and Criteria for Optimization and Validation of Real-Time PCR assays |
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Marijke Raymaekers, Virga Jesse Hospital, Hasselt |
| 10:20 - 10:40 |
Development of a consistent protocol for qPCR analysis in azalea flowers |
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Ellen De Keyser, Institute for Agricultural and Fisheries Research, Melle |
| 10:40 - 11:00 |
Accuracy of sodium bisulphite conversion and real-time PCR for quantitative DNA methylation analysis |
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Ilse Van der Auwera, University of Antwerp |
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| 11:00 - 11:30 |
Coffee break, booth visit |
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Session 2: Biomarkers |
| 11:30 - 11:50 |
Prognostic multigene expression classification of neuroblastoma patients, a SIOPEN study |
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Joëlle Vermeulen, Ghent University |
| 11:50 - 12:10 |
Application of realtime RT-QPCR to develop new biomarkers for soil quality assessment |
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Muriël de Boer, VU University Amsterdam |
| 12:10 - 12:30 |
Gene expression profiles of antioxidative enzymes and related miRNAs as a tool to identify metal-specific effects in A. thaliana |
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Karen Smeets, Hasselt University |
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| 12:30 - 14:00 |
Lunch, poster session, booth visit, lead sponsor talks |
| 13:00 - 13:15 |
New developments as answer to new challenges in Life Science |
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Katrien Royackers, Roche |
| 13:15 - 13:30 |
The new way to study Gene Expression |
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Nathalie Bernard, Applied Biosystems |
| 13:30 - 13:45 |
CFX96TM – How to design an instrument for the way researchers work |
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Marcus Neusser, Bio-Rad Laboratories |
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Session 3: Technical advancements |
| 14:00 - 14:30 |
Towards understanding the role of miRNAs in normal and malignant germ cell development |
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Leendert Looijenga, Rotterdam, the Netherlands (invited speaker) |
| 14:30 - 14:50 |
Quantitative multiplex detection of plant pathogens using a novel ligation probe-based system coupled with universal, high-throughput real-time PCR on OpenArrays |
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Ronald van Doorn, Plant Research International, Wageningen |
| 14:50 - 15:10 |
Significant difference or artefact of the method? The impact of thermocycler performance on results |
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Mary Span, Cyclertest, Landgraaf |
| 15:10 - 15:30 |
Stem-loop RT-PCR miRNA expression profiling using minute amounts of input RNA |
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Pieter Mestdagh, Ghent University |
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| 15:30 - 16:00 |
Coffee break, booth visit |
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Session 4: Bioinformatics |
| 16:00 - 16:20 |
Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data |
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Jan Ruijter, Academic Medical Center, Amsterdam |
| 16:20 - 16:40 |
Discovery of noise-tolerant cancer specific biomarkers |
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Luc Dehaspe, Oncomethylome Sciences, Leuven |
| 16:40 - 17:00 |
High-throughput primer design and in silico assay validation |
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Filip Pattyn, Ghent University (invited speaker) |
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| 17:00 - 17:15 |
Closure and awards |
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| 17:15 - 18:00 |
Drinks (sponsored by Bio-Rad) |